Vascular access for central venous catheter (CVC) placement is technically challenging in children. Ultrasound (US) guidance is recommended for pediatric CVC placement, yet many practitioners rely on imprecise anatomic landmark techniques risking procedure failure due to difficulty mastering US guidance. A novel navigation system provides a visual overlay on real-time US images to depict needle trajectory and tip location during cannulation. We report the first pediatric study assessing feasibility and preliminary safety of using a computer-assisted needle navigation system to aid in central venous access.